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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important position in marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two added splice variants had been claimed but thorough nucleotide sequence info is not really nevertheless available.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally has the epitope recognized through the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This manual outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining through the blocked antibody versus the antibody alone, one can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin household, whose customers are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E varieties a complex with and features like a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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